أطروحة الماجستير للطالبة براءه عبدالله الربابعه

ناقشت بنجاح طالبة الماجستير براءه الربابعة من قسم الصيدلة السريرية اليوم الخميس الموافق 20/8/2015م أطروحة الماجستير بعنوان:

" تقييم الدور المثبط لنمو الخلايا لعقار الاملوديبين على خلايا سرطان القولون والمستقيم "

Evaluation of the antiproliferative effect of amlodipine on colorectal cancer cells

وقد ضمت لجنة المناقشة الدكتور محمد القضاة رئيسا وعضوية كل من الدكتور نزار مهيدات والدكتور بلال الحسين والدكتور محمد خطاطبه والدكتورة عبلة بصول كممتحن خارجي.
باسم صفحة أصدقاء كلية الصيدلة_ في جامعة العلوم والتكنولوجيا الأردنية نبارك للطالبة براءه الربابعة إتمامها درجة الماجستير في الصيدلة السريرية بنجاح متمنين لها مزيدا من التقدم والنجاح.

* للاطلاع على ملخص الدراسة:

Worldwide, colorectal cancer (CRC) is a major cause of mortality and morbidity. As part of the current guidelines: surgery, radiotherapy and chemotherapy with certain targeted therapies are the modality of choice for most of colorectal cancer cases. Unfortunately, chemotherapeutic resistance is still a considerable challenge that has not improved in the last few decades. Calcium channel blockers (CCB) through their interactions with different cellular compartment showed an ancillary effect in various tissue types including antiproliferative effect on tumor cells. Amlodipine, a dihydropyridine CCB, inhibits tumor cell growth both in vitro and in vivo in HT-39 breast cancer and A431 human epidermoid carcinoma cells lines. Amlodipine has shown an antiproliferative effect on human leukemia cell lines including multi-drug resistant cell line. In this study, we evaluated the potential antiproliferative effect of amlodipine, and the possible synergism with irinotecan on colorectal cancer cells. The antiproliferative action of amlodipine was assessed through MTT cell viability assay. Flow cytometric analysis of DNA using propidium iodide method was used to examine the effect of amlodipine on apoptosis and cell cycle distribution. Results showed that amlodipine has significant antiproliferative effect on CRC cells, where G1 cell cycle arrest is partially responsible for this growth inhibitory effect of amlodipine. In comparison to irinotecan, amlodipine induced superior reduction in the number of CRC viable cells. However, combination with irinotecan resulted in partial loss of the antiproliferative effect of amlodipine on CRC cells. These results suggest a potential antitumor action for amlodipine in vitro, which in part mediated by G1 cell cycle arrest in CRC cells