ناقش بنجاح طالب الماجستير محمد العزاني من قسم الصيدلة السريرية اليوم الخميس الموافق 26/5/2016م أطروحة الماجستير بعنوان:
" تقييم تأثير تسكين الألم الناتج
عن تناول محلول السكارين عند الجرذان حديثي الولادة على التعلم والذاكرة في مرحلة البلوغ:
دور الجهاز الافيوني الطبيعي"
Evaluation of the effect
of saccharin-induced analgesia in rat pups on learning and memory during
adulthood: role of endogenous opioid system
وقد ضمت لجنة المناقشة الدكتورة خولة نصير رئيساً وعضوية كل من الأستاذ الدكتور كارم الزعبي والدكتور عمر خابور والدكتور أحمد الحسبان والدكتور أمجد أبو ارميله كممتحن خارجي.
باسم صفحة أصدقاء كلية الصيدلة_ في جامعة العلوم والتكنولوجيا الأردنية نبارك للطالب محمد العزاني إتمامه درجة الماجستير في الصيدلة السريرية بنجاح وتفوق متمنين له مزيدا من التقدم.
للإطلاع على ملخص
الدراسة
Premature infants are usually subjected to multiple painful and
invasive procedures in the neonatal intensive care unit during their first days
of life, these painful procedures are unrecognized and undertreated. Early pain
experience has been associated with long-term consequences such as altered pain
sensitivity, and memory impairment. Several studies have shown that
sweet-tasting solutions given orally produce analgesia by mechanisms not
clearly defined but some suggest a role in the activation of the endogenous
opioid system. Animal models have shown that the acute or chronic oral intake
of saccharin, an artificial sweeting agent, causes analgesia. In this study we
examined the effect saccharin induced analgesia in infant rats on pain
sensitivity and spatial learning and memory during adulthood. Naltrexone, a
pure opioid antagonist, was given orally to evaluate whether saccharin-induce
analgesia is mediated via endogenous opioid system. All treatments were started
on day one of birth and continued for two weeks. Pain in rat pups was induced
via needle prick of the paws. After ten weeks, Radial Arm Water Maze test
(RAWM) test was conducted to assess the hippocampal dependent spatial learning
and memory, as well as hot plate apparatus was used to measure pain threshold.
At the end of behavioral tests animals were sacrificed, hippocampus was
dissected, and the levels of B-endorphins, enkephalin, and brain derived
neurotrophic factor (BDNF) were assessed using ELISA. Our results have shown
that acute repeated neonatal pain had reduced pain threshold,
saccharin/naltrexone administration normalized the pain threshold values
approximately similar to or higher than control tactile group. In addition,
Acute repeated neonatal pain impaired long-term memory, and saccharin or
naltrexone treatments prevented such impairment. Biochemical tests demonstrated
that pain during infancy decreased enkephalins and BDNF levels, pretreatment
with saccharin significantly normalized and increased their level. Naltrexone
reversed such effects on enkephalins, while producing BDNF levels comparable to
control group. In conclusion, Acute repeated neonatal pain impaired long-term
memory during adulthood, and pretreatment with saccharin prevented this
impairment via mechanisms that appear to involve BDNF, most likely through
increased enkephalin levels. Interestingly, naltrexone did not antagonize the
effects of saccharin in pain-induced group on pain threshold, but it augmented
saccharin effects, which may indicate synergistic effects.
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