مناقشة طالب الماجستير عمر اليعقوب من قسم الصيدلة السريرية

ناقش بنجاح طالب الماجستير عمر نصري اليعقوب من قسم الصيدلة السريرية اليوم الخميس الموافق 8/12/2016م أطروحة الماجستير بعنوان:

تقييم التأثير الوقائي للتمبول علی اختلال الإدراك و ضعف الذاكرة الناتج عن اضطراب ما بعد الصدمة

Evaluating the protective effect of tempol on memory and cognitive impairment induced by post traumatic stress disorder

وقد ضمت لجنة المناقشة الأستاذ الدكتور كارم الزعبي رئيساً وعضوية كل من الدكتورة عبير ربابعة والدكتورة نائلة بولاتوفا كممتحن خارجي. باسم صفحة أصدقاء كلية الصيدلة_ في جامعة العلوم والتكنولوجيا الأردنية نبارك للطالب عمر اليعقوب إتمامه درجة الماجستير في الصيدلة السريرية بنجاح وتفوق متمنين له مزيدا من التقدم.

للإطلاع على ملخص الدراسة

Post-traumatic stress disorder (PTSD) is a mental health disorder that can be developed after a terrifying or life threatening event. Many symptoms noticed in patients with PTSD including cognitive and memory impairment. Despite of the different therapeutic approaches used to treat such disorder; studies showed that the response rate for these options approximately between 40-50% of cases only. Tempol is highly efficient membrane-permeable antioxidant, its efficacy in detoxifying reactive oxygen species is numerously shown in cell and animal studies and it also improved both behavioral and cognitive functions in animal models of Alzheimer disease, diabetes and sleep deprivation. In this study we are investigating the protective effect of tempol on Post-traumatic stress disorder induced memory and cognitive impairment on rat model of PTSD. To test this hypothesis we used single prolonged stress (SPS) model (2 h restrain, 20 min forced swimming, 15 min rest, and 1–2 min diethyl ether exposure) as a model of PTSD. Male Wister rats were randomly assigned into four groups: control (provided distilled water), Tempol (provided tempol (80 mg/kg) daily by oral gavage for 4 weeks), PTSD (exposed to SPS and administered distilled water) and Tempol/PTSD (exposed to SPS and administered tempol). We used radial arm water maze (RAWM) to test spatial learning and memory functions and enzyme-linked immunosorbant assay (ELISA) to measure levels of oxidative stress biomarkers and BDNF in the hippocampus. Our results showed that PTSD impaired both short and long term memories, and chronic tempol administration protected against this impairment. Tempol also normalized hippocampal Catalase and SOD activities and increased GSH/GSS ratio and TBARS levels which were all impaired by PTSD. In conclusion, we suggest a protective effect of tempol administration against PTSD –induced short – and long-term memory impairment, and we believe that this protective effect of tempol is accomplished through normalization of oxidative stress in the hippocampus.