أطروحة الماجستير للطالبة هنادي البعول من قسم الصيدلة السريرية

ناقشت بنجاح طالبة الماجستير هنادي فارس البعول من قسم الصيدلة السريرية اليوم الخميس الموافق 8/12/2016م أطروحة الماجستير بعنوان:

تأثير تعرض الجنين لدخان الارجيله على نموذج من الفئران الشابة بعد اثارة حساسية الربو

EFFECT OF PRENATAL WATERPIPE SMOKE ON ALLERGEN DRIVEN MURINE MODEL OF ASTHMA IN ADULT OFFSPRING MICE

وقد ضمت لجنة المناقشة  الدكتورة نور صوالحة رئيساً وعضوية كل من الأستاذ الدكتور كارم الزعبي والدكتورة باسمة المومني والأستاذة الدكتورة عبلة بصول كممتحن خارجي. باسم صفحة أصدقاء كلية الصيدلة_ في جامعة العلوم والتكنولوجيا الأردنية نبارك للطالبة هنادي البعول إتمامها درجة الماجستير في الصيدلة السريرية بنجاح وتفوق متمنين لها مزيدا من التقدم.

للإطلاع على ملخص الدراسة

Introduction:Asthma is a chronic inflammatory airway disease. Asthma is triggered by many allergens such as smoking. Worldwide,around one billion individual smokes regularly including women. Recently, waterpipe tobacco smoking becomes an international trend of smoking in women especially pregnant women.Prenatal tobaccoexposure resulted in several detrimental effects to offspring's. The aims of this study were to evaluate the effect of prenatal waterpipe smoking on pregnancy outcomes, airway inflammation and oxidative stress level in allergen driven murine model of asthma in adult offspring mice.

Method: 16 pregnant BALB/c female mice were used in the experiment; 8 of them were exposed to fresh air (control) and the others were exposed to waterpipe tobacco smoke (WTS) for 2 hours per day for the whole period of pregnancy. After delivery, the offspring mice were divided into 4 groups. Asthma mice were sensitized and challenged with ovalbumin. Bronchoalveolar lavage fluid (BALF) was analyzed to measure the total and differential inflammatory cells count. Lung tissues homogenate were used to measure the level of inflammatory cytokines [interleukin (IL)- 2, 10 and 18] and oxidative stress markers [superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and thiobarbituric acid reactive substances (TBARS)].

Results:prenatal WTS exposure significantly reduce mothers weight during pregnancy, offspring weight and length at birth as well as offspring weight gain. In addition prenatal WTS exposure increased the level of airway recruitment of inflammatory cells, level of lung IL-10, SOD and catalase in offspring as compared to unexposed offspring (P<0.05). Also, prenatal WTS exposure enhanced airway inflammation in response to allergen, as noticed by the increased level of airway inflammatory cells, lung levels of SOD and catalase as well as reduced levels of IL-10 as compared to offspring mice that were exposed to allergen (P<0.05).  The lung levels of GPx and TBARS were not affected by prenatal WTS exposure (P>0.05). 

Conclusion: Prenatal exposure to WTS is associated with adverse effects on pregnancy outcomes. In addition, maternal WTS increased the level of airway inflammation and disturbed the level of anti-oxidant enzymes in adult offspring mice. Maternal exposure to WTS enhanced allergen induced airway inflammation in adult offspring mice.